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Product Name
PRKACB antibody
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Description
PRKACB Rabbit Polyclonal antibody. Positive IHC detected in human medulloblastoma tissue. Positive IF detected in Hela cells. Positive IP detected in mouse brain tissue. Positive WB detected in HT-1080 cells, A549 cells, MCF7 cells, mouse brain tissue, PC-3 cells. Observed molecular weight by Western-blot: 46-55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp781I2452 antibody; PKA C beta antibody; PKACB antibody; PRKACB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRKACB recombinant protein (Accession Number: NM_207578). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HT-1080 cells were subjected to SDS PAGE followed by western blot with Catalog No:114194(PRKACB antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human medulloblastoma using Catalog No:114194(PRKACB antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using PRKACB antibody Catalog No:114194 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PRKACB (IP:Catalog No:114194, 4ug; Detection:Catalog No:114194 1:500) with mouse brain tissue lysate 2640ug.
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Background
PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
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