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PREP antibody

Cat.#: 114184

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Product Information

  • Product Name
    PREP antibody
  • Documents
  • Description
    PREP Rabbit Polyclonal antibody. Positive IHC detected in human gliomas. Positive WB detected in human lung tissue, Jurkat cells, mouse lung tissue. Observed molecular weight by Western-blot: 81kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    human, mouse, rat; other species not tested.
  • Alternative names
    PREP antibody; PEP antibody; Post proline cleaving enzyme antibody; prolyl endopeptidase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PREP recombinant protein (Accession Number: NM_002726). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    human lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:114184(PREP antibody) at dilution of 1:400

    human lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:114184(PREP antibody) at dilution of 1:400

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114184(PREP antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114184(PREP antibody) at dilution of 1:50 (under 10x lens)

  • Background
    PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)
  • References
    • Braber S, Koelink PJ, Henricks PA. Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown. American journal of physiology. Lung cellular and molecular physiology. 300(2):L255-65. 2011.
    • Overbeek SA, Braber S, Koelink PJ. Cigarette smoke-induced collagen destruction; key to chronic neutrophilic airway inflammation? PloS one. 8(1):e55612. 2013.
    • Koelink PJ, Overbeek SA, Braber S. Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease. Gut. 63(4):578-87. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"