PRDX6 antibody

Cat.#: 114178

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Product Information

  • Product Name
    PRDX6 antibody
  • Documents
  • Description
    PRDX6 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in A549 cells, HeLa cells, SH-SY5Y cells. Positive IF detected in Hela cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 25-30kd
  • Tested applications
    ELISA, WB, IF, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    1 Cys antibody; 1 Cys peroxiredoxin antibody; 1 Cys PRX antibody; 24 kDa protein antibody; aiPLA2 antibody; Antioxidant protein 2 antibody; AOP2 antibody; KIAA0106 antibody; Liver 2D page spot 40 antibody; NSGPx antibody; p29 antibody; peroxiredoxin 6 antibody; PRDX6 antibody; PRX antibody; Red blood cells page spot 12 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PRDX6 recombinant protein (Accession Number: NM_004905). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:114178(PRDX6 antibody) at dilution of 1:500

    A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:114178(PRDX6 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human brain using Catalog No:114178(PRDX6 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:114178(PRDX6 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:114178(PRDX6 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:114178(PRDX6 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of Hela cells, using PRDX6 antibody Catalog No:114178 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of Hela cells, using PRDX6 antibody Catalog No:114178 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-PRDX6 (IP:Catalog No:114178, 3ug; Detection:Catalog No:114178 1:500) with HeLa cells lysate 2800ug.

    IP Result of anti-PRDX6 (IP:Catalog No:114178, 3ug; Detection:Catalog No:114178 1:500) with HeLa cells lysate 2800ug.

  • Background
    Prdx6(Peroxiredoxin-6) , a unique member of the peroxiredoxin family of antioxidants, is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides(PMID: 17382207).It is also named as AOP2, KIAA0106. It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung(PMID:15890616).
  • References
    • Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI. MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. Mitochondrion. 20:43-51. 2015.
    • Ma S, Zhang X, Zheng L. Peroxiredoxin 6 Is a Crucial Factor in the Initial Step of Mitochondrial Clearance and Is Upstream of the PINK1-Parkin Pathway. Antioxidants & redox signaling. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"