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Product Name
PPP3CB-specific antibody
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Description
PPP3CB-specific Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IHC detected in human heart tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, A431 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 57-59 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CALNA2 antibody; CALNB antibody; CAM PRP catalytic subunit antibody; CNA2 antibody; PPP3CB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_021132). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114111(PPP3CB antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human heart using Catalog No:114111(PPP3CB antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:114111(PPP3CB antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PPP3CB (IP:Catalog No:114111, 4ug; Detection:Catalog No:114111 1:500) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed A431 cells using Catalog No:114111(PPP3CB Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PPP3CB, also named as CALNA2, CALNB and CNA2, belongs to the PPP phosphatase family and PP-2B subfamily. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CB.
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References
- Wu J, Zheng C, Wang X. MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes. The Journal of clinical investigation. 125(11):4091-106. 2015.
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