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Product Name
PPP3CA antibody
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Description
PPP3CA Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse skeletal muscle tissue, mouse heart tissue, SH-SY5Y cells. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 61 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CALN antibody; CALNA antibody; CALNA1 antibody; CAM PRP catalytic subunit antibody; CCN1 antibody; CNA antibody; CNA1 antibody; PPP2B antibody; PPP3CA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPP3CA recombinant protein (Accession Number: NM_001130691). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:114108(PPP3CA antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:114108(PPP3CA antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:114108(PPP3CA antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PPP3CA (IP:Catalog No:114108, 4ug; Detection:Catalog No:114108 1:1000) with mouse heart tissue lysate 4400ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed SH-SY5Y cells using Catalog No:114108(PPP3CA Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PPP3CA represents protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
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