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Product Name
PPP2R2B antibody
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Description
PPP2R2B Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, MCF7 cells. Positive IP detected in SH-SY5Y cells. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B55 BETA antibody; FLJ95686 antibody; PP2A B55BETA antibody; PP2A PR55B antibody; PP2A subunit B isoform beta antibody; PP2A subunit B isoform R2 beta antibody; PP2AB BETA antibody; PP2APR55 BETA antibody; PPP2R2B antibody; PR2AB BETA antibody; PR2AB55 BETA antibody; PR2APR55 BETA antibody; PR52B antibody; PR55 BETA antibody; SCA12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPP2R2B recombinant protein (Accession Number: NM_181675). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:114100(PPP2R2B antibody) at dilution of 1:500
IP Result of anti-PPP2R2B (IP:Catalog No:114100, 4ug; Detection:Catalog No:114100 1:500) with SH-SY5Y cells lysate 1200ug.
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:114100(PPP2R2B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival.(BioFormosa（2009）44(2): 77-84). It has 5 isoforms produced by alternative splicing.
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