-
Product Name
PPM1B antibody
- Documents
-
Description
PPM1B Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human skeletal muscle tissue. Positive WB detected in MCF7 cells. Observed molecular weight by Western-blot: 50-53 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
PP2C beta antibody; PP2C beta X antibody; PP2CB antibody; PP2CBETA antibody; PPC2BETAX antibody; PPM1B antibody; Protein phosphatase 1B antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PPM1B recombinant protein (Accession Number: NM_177969). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:114133(PPM1B antibody) at dilution of 1:400
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:114133(PPM1B Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:114133(PPM1B Antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed MCF-7 cells using Catalog No:114133(PPM1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
PPM1B represents protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"