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Product Name
PPIL1 antibody
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Description
PPIL1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, A375 cells, HeLa cells. Positive IP detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 18 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 124 antibody; CYPL1 antibody; hCyPX antibody; PPIase antibody; PPIL1 antibody; Rotamase PPIL1 antibody; UNQ2425/PRO4984 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPIL1 recombinant protein (Accession Number: NM_016059). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114127(PPIL1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114127(PPIL1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114127(PPIL1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PPIL1 (IP:Catalog No:114127, 3ug; Detection:Catalog No:114127 1:400) with NIH/3T3 cells lysate 1600ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:114127(PPIL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PPIL1 represents peptidylprolyl isomerase (cyclophilin)-like 1
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