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Product Name
POSH antibody
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Description
POSH Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive WB detected in mouse brain tissue, DU 145 cells, HEK-293 cells, human brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 93-100 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1494 antibody; Plenty of SH3s antibody; POSH antibody; Protein POSH antibody; RING finger protein 142 antibody; RNF142 antibody; SH3 multiple domains protein 2 antibody; SH3MD2 antibody; SH3RF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POSH recombinant protein (Accession Number: BC053671). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114064(SH3RF1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114064(SH3RF1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114064(SH3RF1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SH3RF1 (IP:Catalog No:114064, 4ug; Detection:Catalog No:114064 1:1000) with mouse brain tissue lysate 4000ug.
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Background
POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains. Recently POSG has been identified as a novel nexus linking activity of TGF-β and JNK signaling via TAK1 (TGF-β-activated kinase 1) and a regulator of synaptic growth responses. (25800055)
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References
- West RJ, Lu Y, Marie B, Gao FB, Sweeney ST. Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia. The Journal of cell biology. 208(7):931-47. 2015.
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