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Product Name
POLR3G antibody
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Description
POLR3G Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells. Observed molecular weight by Western-blot: 26-30 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
POLR3G antibody; RNA polymerase III subunit C7 antibody; RPC32 antibody; RPC7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLR3G recombinant protein (Accession Number: XM_011543100). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114052(POLR3G Antibody) at dilution of 1:1000
IP Result of anti-POLR3G (IP:Catalog No:114052, 3ug; Detection:Catalog No:114052 1:500) with HeLa cells lysate 2800ug.
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Background
POLR3G also named as RNA polymerase III subunit C7 is a 223 amino acid protein, which belongs to eukaryotic RPC7 RNA polymerase subunit family. The molecular weight of POLR3G is 26 kDa. POLR3G localizes in the nucleus and is a component of the RNA polymerase III complex, which may be involved either in the recruitment and stablization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.
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