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Product Name
POLR2J antibody
- Documents
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Description
POLR2J Rabbit Polyclonal antibody. Positive WB detected in A549 cells, HeLa cells, human brain tissue. Positive IF detected in A549 cells. Positive IHC detected in human heart tissue. Observed molecular weight by Western-blot: 13 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hRPB14 antibody; POLR2J antibody; POLR2J1 antibody; RPB11 antibody; RPB11A antibody; RPB11m antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLR2J recombinant protein (Accession Number: NM_006234). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:114045(POLR2J antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human heart using Catalog No:114045(POLR2J antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using Catalog No:114045(POLR2J Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
POLR2J represents polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"