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Product Name
POLR2C antibody
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Description
POLR2C Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in human kidney tissue, HeLa cells, mouse kidney tissue. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hRPB33 antibody; hsRPB3 antibody; POLR2C antibody; RNA polymerase II subunit 3 antibody; RNA polymerase II subunit B3 antibody; RPB3 antibody; RPB31 antibody; RPB33 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLR2C recombinant protein (Accession Number: NM_032940). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:114040(POLR2C antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:114040(POLR2C antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:114040(POLR2C antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-POLR2C (IP:Catalog No:114040, 4ug; Detection:Catalog No:114040 1:500) with HeLa cells lysate 2000ug.
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Background
POLR2C represents polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
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