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Product Name
PNPO antibody
- Documents
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Description
PNPO Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive IF detected in Hela cells. Positive WB detected in MCF7 cells, human brain tissue, human heart tissue, human liver tissue. Observed molecular weight by Western-blot: 30kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ10535 antibody; PDXPO antibody; PNPO antibody; Pyridoxamine phosphate oxidase antibody; Pyridoxine 5 phosphate oxidase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PNPO recombinant protein (Accession Number: NM_018129). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113983(PNPO antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:113983(PNPO antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:113983(PNPO antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using PNPO antibody Catalog No:113983 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
PNPO represents pyridoxamine 5'-phosphate oxidase
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