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Product Name
PNPLA6 antibody
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Description
PNPLA6 Rabbit Polyclonal antibody. Positive IP detected in N/A. Positive WB detected in mouse brain tissue, HEK-293 cells, SH-SY5Y cells. Positive IF detected in HepG2 cells, MCF-7 cells. Observed molecular weight by Western-blot: 150kd
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Neuropathy target esterase antibody; NTE antibody; NTEMND antibody; PNPLA6 antibody; SPG39 antibody; sws antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PNPLA6 recombinant protein (Accession Number: NM_001166113). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: N/AIF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113982(PNPLA6 antibody) at dilution of 1:500
IP result of anti-PNPLA6 (Catalog No:113982 for IP and Detection) with 293 cell.
Immunofluorescent analysis of HepG2 cells, using PNPLA6 antibody Catalog No:113982 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing.
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References
- Ascano M, Mukherjee N, Bandaru P. FMRP targets distinct mRNA sequence elements to regulate protein expression. Nature. 492(7429):382-6. 2012.
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