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Product Name
PLSCR1 antibody
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Description
PLSCR1 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue, human kidney tissue, human pancreas tissue, human spleen tissue. Positive IF detected in HeLa cells. Positive FC detected in HeLa cells. Positive WB detected in human skeletal muscle tissue, HEK-293 cells, human lung tissue, mouse lung tissue, mouse skeletal muscle tissue. Positive IP detected in mouse lung tissue. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IHC, FC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MMTRA1B antibody; phospholipid scramblase 1 antibody; PL scramblase 1 antibody; PLSCR1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PLSCR1 recombinant protein (Accession Number: NM_021105). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:114012(PLSCR1 antibody) at dilution of 1:2000
Immunofluorescent analysis of HeLa cells using Catalog No:114012(PLSCR1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114012(PLSCR1 antibody) at dilution of 1:100 (under 10x lens)
1X10^6 HeLa cells were stained with 0.2ug PLSCR1 antibody (Catalog No:114012, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-PLSCR1 (IP:Catalog No:114012, 4ug; Detection:Catalog No:114012 1:500) with mouse lung tissue lysate 2800ug.
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Background
PLSCR1 represents phospholipid scramblase 1
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References
- Gong Q, Cheng M, Chen H. Phospholipid scramblase 1 mediates hepatitis C virus entry into host cells. FEBS letters. 585(17):2647-52. 2011.
- Yuan Y, Tian C, Gong Q. Interactome map reveals phospholipid scramblase 1 as a novel regulator of hepatitis B virus x protein. Journal of proteome research. 14(1):154-63. 2015.
- Berghold VM, Gauster M, Hemmings DG. Phospholipid scramblase 1 (PLSCR1) in villous trophoblast of the human placenta. Histochemistry and cell biology. 143(4):381-96. 2015.
- Slone EA, Pope MR, Fleming SD. Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation. Journal of leukocyte biology. 98(5):791-804. 2015.
- Kuo YB, Chan CC, Chang CA. Identification of phospholipid scramblase 1 as a biomarker and determination of its prognostic value for colorectal cancer. Molecular medicine (Cambridge, Mass.). 17(1-2):41-7. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"