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Product Name
PLS3 antibody
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Description
PLS3 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, COLO 320 cells, HeLa cells. Positive IHC detected in human oesophagus. Observed molecular weight by Western-blot: 63kd,38kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
PLS3 antibody; T plastin antibody; plastin 3 (T isoform) antibody; Plastin 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005032). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114010(PLS3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human oesophagus using Catalog No:114010(PLS3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human oesophagus using Catalog No:114010(PLS3 antibody) at dilution of 1:50 (under 40x lens)
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Background
PLS3, also named as T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. Both T- and L-plastin are implicated in several diseases, L-plastin is considered to be a valuable marker for cancer. (PMID: 15960882) This antibody is specific to PLS3
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