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Product Name
PLK1-phospho(S326) antibody
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Description
PLK1-phospho(S326) Rabbit Polyclonal antibody. Positive IHC detected in human colon tissue. Positive WB detected in MCF-7 cells. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PLK antibody; PLK 1 antibody; PLK1 antibody; Polo like kinase 1 antibody; STPK13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005030). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113968(PLK1-phospho antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human colon using Catalog No:113968(PLK1-phospho antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon using Catalog No:113968(PLK1-phospho antibody) at dilution of 1:50 (under 40x lens)
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Background
PLK1, also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. The antibody is specific to Ser(326)-phospho.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"