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Product Name
PLEKHO2 antibody
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Description
PLEKHO2 Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IHC detected in human lung tissue, human cervical cancer tissue, human spleen tissue. Positive WB detected in HeLa cells, mouse thymus tissue. Observed molecular weight by Western-blot: 53 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp761K2312 antibody; FLJ38884 antibody; PLEKHO2 antibody; PLEKHQ1 antibody; PP1628 antibody; pp9099 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PLEKHO2 recombinant protein (Accession Number: NM_001195059). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113965(PLEKHO2 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:113965(PLEKHO2 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human lung tissue slide using Catalog No:113965(PLEKHO2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A431 cells using Catalog No:113965(PLEKHO2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Pleckstrin homology domain-containing proteins family O member 2 (PLEKHO2)’s function remains largely unknown. Genetic polymorphisms of PLEKHO2 contribute to diabetic retinopathy (DR) development. Functional prediction analysis strengthened the likelihood of participation of PLEKHO2 in the development of DR (Eric C. Han et al., 2012).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"