-
Product Name
PLEK antibody
- Documents
-
Description
PLEK Rabbit Polyclonal antibody. Positive WB detected in U-937 cells, rat spleen tissue. Positive IP detected in U-937 cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 45-47 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
FLJ27168 antibody; P47 antibody; Platelet 47 kDa protein antibody; pleckstrin antibody; PLEK antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PLEK recombinant protein (Accession Number: NM_002664). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:113953(PLEK antibody) at dilution of 1:1000
IP Result of anti-PLEK (IP:Catalog No:113953, 3ug; Detection:Catalog No:113953 1:1000) with U-937 cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113953(PLEK antibody) at dilution of 1:50 (under 40x lens)
-
Background
PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"