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Product Name
PLAP antibody
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Description
PLAP Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in human placenta tissue, HepG2 cells, mouse liver tissue. Positive IHC detected in human placenta tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ALP antibody; ALPP antibody; FLJ61142 antibody; PALP antibody; Placental alkaline phosphatase antibody; PLAP antibody; PLAP 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PLAP recombinant protein (Accession Number: NM_001632). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:113939(ALPP Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human placenta using Catalog No:113939(ALPP antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:113939(ALPP antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ALPP (IP:Catalog No:113939, 4ug; Detection:Catalog No:113939 1:700) with HepG2 cells lysate 1800ug.
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Background
Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.
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