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Product Name
PKC gamma antibody
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Description
PKC gamma Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human cerebellum tissue, human gliomas tissue, rat brain tissue. Positive WB detected in human brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 76-80 kDa
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Tested applications
ELISA, WB, FC, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PKC gamma antibody; PKCC antibody; PKCG antibody; PRKCG antibody; Protein kinase C gamma type antibody; protein kinase C antibody; gamma antibody; SCA14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PKC gamma recombinant protein (Accession Number: NM_002739). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113851(PRKCG antibody) at dilution of 1:1000
IP Result of anti-PRKCG (IP:Catalog No:113851, 4ug; Detection:Catalog No:113851 1:500) with mouse brain tissue lysate 2640ug.
Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using Catalog No:113851(PRKCG Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using Catalog No:113851(PRKCG Antibody) at dilution of 1:200 (under 40x lens).
1X10^6 HeLa cells were stained with 1ug PRKCG antibody (Catalog No:113851, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.
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References
- Zheng Y, Yin X, Huo F, Xiong H, Mei Z. Analgesic effects and possible mechanisms of iridoid glycosides from Lamiophlomis rotata (Benth.) Kudo in rats with spared nerve injury. Journal of ethnopharmacology. 173:204-11. 2015.
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