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Product Name
PJA1 antibody
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Description
PJA1 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 71-80 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PJA1 antibody; praja ring finger 1 antibody; Praja1 antibody; RING finger protein 70 antibody; RNF70 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PJA1 recombinant protein (Accession Number: NM_001382775). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113843(PJA1 antibody) at dilution of 1:200
IP Result of anti-PJA1 (IP:Catalog No:113843, 3ug; Detection:Catalog No:113843 1:300) with mouse brain tissue lysate 3600ug.
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Background
PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.
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