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Product Name
PITX1 antibody
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Description
PITX1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human tonsillitis tissue, human colon tissue. Positive WB detected in HeLa cells, A431 cells, MCF7 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 38-40 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BFT antibody; Homeobox protein PITX1 antibody; paired like homeodomain 1 antibody; Pituitary homeobox 1 antibody; PITX1 antibody; POTX antibody; PTX1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PITX1 recombinant protein (Accession Number: NM_002653). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113840(PITX1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:113840(PITX1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:113840(PITX1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PITX1 antibody Catalog No:113840 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PITX1 (IP:Catalog No:113840, 4ug; Detection:Catalog No:113840 1:1000) with HeLa cells lysate 1200ug.
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Background
PITX1, also named as Pituitary homeobox 1 or BFT, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. Bicoid subfamily. PITX1 localizes in the nucleus and may play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
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References
- Wang YG, Li XD, Liu ZY. All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression. Toxicology letters. 224(2):282-9. 2014.
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