-
Product Name
PITRM1 antibody
- Documents
-
Description
PITRM1 Rabbit Polyclonal antibody. Positive IP detected in A549 cells. Positive WB detected in human placenta tissue, A549 cells, mouse testis tissue. Observed molecular weight by Western-blot: 110-117 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
hMP1 antibody; hPreP antibody; KIAA1104 antibody; Metalloprotease 1 antibody; MP1 antibody; pitrilysin metallopeptidase 1 antibody; Pitrilysin metalloproteinase 1 antibody; PITRM1 antibody; PreP antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PITRM1 recombinant protein (Accession Number: BC001150). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
-
Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:113839(PITRM1 antibody) at dilution of 1:300
IP Result of anti-PITRM1 (IP:Catalog No:113839, 4ug; Detection:Catalog No:113839 1:500) with A549 cells lysate 2000ug.
-
Background
PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.This antibody is specific to PITRM1.
-
References
- Mitchell AJ, Lone AM, Tinoco AD, Saghatelian A. Proteolysis controls endogenous substance P levels. PloS one. 8(7):e68638. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"