Product Name

PISD antibody

Documents

Description

PISD Rabbit Polyclonal antibody. Positive IP detected in L02 cells. Positive WB detected in K-562 cells, COLO 320 cells, HeLa cells, HepG2 cells, human kidney tissue, human spleen tissue, L02 cells, NIH/3T3 cells. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 50~55kd

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DJ858B16 antibody; dJ858B16.2 antibody; DKFZp566G2246 antibody; PISD antibody; PSDC antibody; PSSC antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of PISD recombinant protein (Accession Number: NM_178022). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

Validations

K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113835(PISD antibody) at dilution of 1:300

Immunohistochemical of paraffin-embedded human kidney using Catalog No:113835(PISD antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human kidney using Catalog No:113835(PISD antibody) at dilution of 1:100 (under 40x lens)

IP Result of anti-PISD (IP:Catalog No:113835, 3ug; Detection:Catalog No:113835 1:500) with L02 cells lysate 1400ug.

Background

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.This antibody is specific to PISD. The sequence correspond to the beta isoform.

PISD antibody

Product Information

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