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Product Name
PIK3C2A antibody
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Description
PIK3C2A Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human heart tissue, human placenta tissue. Positive IP detected in PC-3 cells. Positive WB detected in PC-3 cells, HeLa cells, human placenta tissue. Observed molecular weight by Western-blot: 191 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CPK antibody; DKFZp686L193 antibody; PI 3 Kinase Class 2A antibody; PI 3-kinase C2alpha antibody; PI3 K C2(ALPHA) antibody; PI3 K C2A antibody; PI3K C2 alpha antibody; PIK3C2A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIK3C2A recombinant protein (Accession Number: XM_017017924). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113901(PIK3C2A antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human heart using Catalog No:113901(PIK3C2A antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113901(PIK3C2A antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PIK3C2A (IP:Catalog No:113901, 4ug; Detection:Catalog No:113901 1:1000) with PC-3 cells lysate 3200ug.
Immunofluorescent analysis of HeLa cells using Catalog No:113901(PIK3C2A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PIK3C2A represents phosphoinositide-3-kinase, class 2, alpha polypeptide
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