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Product Name
PIH1D1 antibody
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Description
PIH1D1 Rabbit Polyclonal antibody. Positive IHC detected in human lung tissue, human ovary tissue. Positive IF detected in Hela cells. Positive WB detected in HeLa cells, A431 cells, human testis tissue, human uterus tissue, mouse eye tissue, mouse ovary tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20643 antibody; NOP17 antibody; Nucleolar protein 17 homolog antibody; PIH1 domain containing 1 antibody; PIH1D1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIH1D1 recombinant protein (Accession Number: NM_017916). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113900(PIH1D1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human lung using Catalog No:113900(PIH1D1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:113900(PIH1D1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using PIH1D1 antibody Catalog No:113900 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-PIH1D1 (IP:Catalog No:113900, 4ug; Detection:Catalog No:113900 1:1000) with HeLa cells lysate 1200ug.
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Background
PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.
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References
- Machado-Pinilla R, Liger D, Leulliot N, Meier UT. Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs. RNA (New York, N.Y.). 18(10):1833-45. 2012.
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