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Product Name
PI3K p110(alpha) antibody
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Description
PI3K p110(alpha) Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, Jurkat cells. Positive IHC detected in human lung cancer tissue, human breast cancer tissue. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
p110 alpha antibody; PI3 kinase subunit alpha antibody; PI3K antibody; PI3K alpha antibody; PI3K p110(alpha) antibody; PI3-kinase p110 subunit alpha antibody; PIK3CA antibody; PtdIns 3 kinase subunit alpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PI3K p110(alpha) recombinant protein (Accession Number: XM_006713658). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113871(PIK3CA antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113871(PIK3CA antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113871(PIK3CA antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PIK3CA (IP:Catalog No:113871, 4ug; Detection:Catalog No:113871 1:800) with mouse brain tissue lysate 2560ug.
1X10^6 Jurkat cells were stained with 0.5ug PIK3CA antibody (Catalog No:113871, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB). The antibody is specific to PIK3CA.
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References
- Tian L, Li L, Xing W. IRGM1 enhances B16 melanoma cell metastasis through PI3K-Rac1 mediated epithelial mesenchymal transition. Scientific reports. 5:12357. 2015.
- Wu ZH, Tao ZH, Zhang J. MiRNA-21 induces epithelial to mesenchymal transition and gemcitabine resistance via the PTEN/AKT pathway in breast cancer. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"