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Product Name
PI3K p101 antibody
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Description
PI3K p101 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue, human liver cancer tissue, human lung cancer tissue. Positive WB detected in rat lymph tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
F730038I15Rik antibody; FOAP 2 antibody; p101 antibody; P101 PI3K antibody; PI3 kinase p101 subunit antibody; PI3K p101 antibody; PIK3R5 antibody; Protein FOAP 2 antibody; PtdIns 3 kinase p101 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PI3K p101 recombinant protein (Accession Number: NM_001251852). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
rat lymph tissue were subjected to SDS PAGE followed by western blot with Catalog No:113869(PIK3R5 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using PIK3R5 antibody Catalog No:113869 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:113869(PIK3R5 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:113869(PIK3R5 Antibody) at dilution of 1:50 (under 40x lens)
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Background
PIK3R5 represents phosphoinositide-3-kinase, regulatory subunit 5
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References
- Biethahn K, Orinska Z, Vigorito E. miRNA-155 controls mast cell activation by regulating the PI3Kγ pathway and anaphylaxis in a mouse model. Allergy. 69(6):752-62. 2014.
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