-
Product Name
PHF19 antibody
- Documents
-
Description
PHF19 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells, Mouse ES cells. Positive WB detected in rat brain tissue, mouse ES cells and 293 cells. Positive IF detected in Mouse ES cells. Observed molecular weight by Western-blot: 66 kDa
-
Tested applications
ELISA, IP, IF, WB
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
hPCL3 antibody; MTF2L1 antibody; PCL3 antibody; PHD finger protein 19 antibody; PHF19 antibody; Polycomb like protein 3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PHF19 recombinant protein (Accession Number: NM_001009936). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IF: 1:20-1:200
-
Validations
IP result of anti-PHF19(Catalog No:113810) in Mouse ES cell from Monika.
IP Result of anti-PHF19 (IP:Catalog No:113810, 4ug; Detection:Catalog No:113810 1:500) with HeLa cells lysate 2000ug.
IF result of Mouse ES cell From Kevin Corbit.
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113810(PHF19 Antibody) at dilution of 1:300
-
Background
PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.
-
References
- Hunkapiller J, Shen Y, Diaz A. Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal. PLoS genetics. 8(3):e1002576. 2012.
- Cai L, Rothbart SB, Lu R. An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting. Molecular cell. 49(3):571-82. 2013.
- Holdt LM, Hoffmann S, Sass K. Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks. PLoS genetics. 9(7):e1003588. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"