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Product Name
PHD2 antibody
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Description
PHD2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas tissue, human heart tissue. Positive WB detected in HEK-293 cells, mouse pancreas tissue. Observed molecular weight by Western-blot: 46kd,36kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C1orf12 antibody; DKFZp761F179 antibody; ECYT3 antibody; Egl nine homolog 1 antibody; EGLN1 antibody; HIF PH2 antibody; HIF prolyl hydroxylase 2 antibody; HIFPH2 antibody; HPH 2 antibody; HPH2 antibody; PHD2 antibody; PNAS 137 antibody; SM 20 antibody; SM20 antibody; ZMYND6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_022051). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113804(EGLN1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:113804(EGLN1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:113804(EGLN1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using EGLN1 antibody Catalog No:113804 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). The antibody is specific to EGLN1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"