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Product Name
PFN2 antibody
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Description
PFN2 Mouse Monoclonal antibody. Positive WB detected in mouse brain tissue, rat brain tissue. Positive IHC detected in human kidney tissue, human lung cancer tissue, human skeletal muscle tissue, mouse brain tissue. Observed molecular weight by Western-blot: 15 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
D3S1319E antibody; PFL antibody; PFN2 antibody; Prifilin 2 antibody; PROF2 antibody; profilin 2 antibody; Profilin II antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of PFN2 recombinant protein (Accession Number: NM_053024). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107466(PFN2 Antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:107466(PFN2 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:107466(PFN2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
PFN2 (profilin 2) is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. The MW of this protein is 15 kDa, and this antibody specially recognises the 15 kDa protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"