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Product Name
PFKL antibody
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Description
PFKL Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 85 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686G1648 antibody; DKFZp686L2097 antibody; FLJ30173 antibody; FLJ40909 antibody; PFK B antibody; PFKL antibody; Phosphofructokinase 1 antibody; phosphofructokinase antibody; liver antibody; Phosphohexokinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PFKL recombinant protein (Accession Number: NM_002626). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113771(PFKL antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:113771(PFKL antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:113771(PFKL antibody) at dilution of 1:50 (under 40x lens)
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Background
PFKL(6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK)(ATP:D-fructose-6-phosphate-1-phosphotransferase).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings,PFK exsits as a system of three of subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs(PMID: 2139864). It has 2 isoforms produced by alternative splicing.
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References
- Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
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