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Product Name
PERK antibody
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Description
PERK Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IHC detected in human pancreas tissue, human placenta tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 125 kDa, 140 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp781H1925 antibody; EIF2AK3 antibody; HRI antibody; HsPEK antibody; Pancreatic eIF2 alpha kinase antibody; PEK antibody; PERK antibody; WRS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PERK recombinant protein (Accession Number: NM_004836). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemistry of paraffin-embedded human pancreas slide using Catalog No:113717(EIF2AK3 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human pancreas slide using Catalog No:113717(EIF2AK3 Antibody) at dilution of 1:50
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113717(EIF2AK3 Antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells using Catalog No:113717(EIF2AK3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PERK is also named as PEK, EIF2AK3 (Eukaryotic translation initiation factor 2-alpha kinase 3) and belongs to the GCN2 subfamily. It acts potentially as a metabolic sensor in the insulin-secreting beta-cells to modulate the trafficking and quality control of proinsulin in the ER relative to the physiological demands for circulating insulin(PMID:20530744).PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases(PMID: 21638082). The full length protein has a signal peptide and a glycosylation site.
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References
- Xu D, Su C, Song X. Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release. Chemical research in toxicology. 28(6):1326-37. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"