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Product Name
Pepsinogen I antibody
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Description
Pepsinogen I Rabbit Polyclonal antibody. Positive IHC detected in human stomach cancer tissue. Positive WB detected in SGC-7901 cells. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Pepsin A antibody; pepsinogen 5 antibody; group I (pepsinogen A) antibody; Pepsinogen I antibody; PGA3 antibody; PGA4 antibody; PGA5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Pepsinogen I recombinant protein (Accession Number: NM_014224). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:113708(PGA5 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:113708(PGA5 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:113708(PGA5 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Pepsinogen I belongs to the peptidase A1 family. It shows particularly broad specificity and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The gene endoces a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. This antibody can recognize PGA3(pepsinogen 3), PGA4(pepsinogen 4), PGA5(pepsinogen 5).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"