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Product Name
PDLIM7 antibody
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Description
PDLIM7 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in SKOV-3 cells, HeLa cells, MCF-7 cells. Positive IHC detected in human ovary tumor tissue. Positive IF detected in SKOV-3 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ENIGMA antibody; LIM mineralization protein antibody; LMP antibody; PDLIM7 antibody; PDZ and LIM domain 7 (enigma) antibody; PDZ and LIM domain protein 7 antibody; Protein enigma antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDLIM7 recombinant protein (Accession Number: NM_005451). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: N/AIHC: 1:20-1:200
IF: 1:20-1:200
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Validations
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113633(PDLIM7,LMP1 antibody) at dilution of 1:2000
IP result of PDLIM7 antibody (Catalog No:113633 for IP and Detection) with HeLa cell lysate.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113633(PDLIM7,LMP1 antibody) at dilution of 1:100 (under 25x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed SKOV-3 cells using Catalog No:113633(PDLIM7,LMP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.
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References
- Liu H, Pan H, Yang H. LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 33(3):294-303. 2015.
- Pan H, Li X, Wang J. LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2-Mediated Osteogenesis Through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 30(8):1523-35. 2015.
- Wang X, Zhang Q, Chen Z, Zhang L. Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions. Journal of endodontics. 34(2):143-7. 2008.
- Fang P, Wang X, Zhang L, Yuan G, Chen Z, Zhang Q. Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development. Journal of molecular histology. 41(4-5):199-203. 2010.
- Zhu Z, Liu Z, Liu J, Bi M, Yang T, Wang J. Proteomic profiling of human placenta-derived mesenchymal stem cells upon transforming LIM mineralization protein-1 stimulation. Cytotechnology. 67(2):285-97. 2015.
- Liu H, Huang L, Zhang Z. LIM mineralization protein-1 inhibits the malignant phenotypes of human osteosarcoma cells. International journal of molecular sciences. 15(4):7037-48. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"