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Product Name
PDLIM5 antibody
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Description
PDLIM5 Rabbit Polyclonal antibody. Positive IP detected in A549 cells. Positive WB detected in HeLa cells, A549 cells, human liver tissue, NIH/3T3. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 63-68 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ENH antibody; ENH1 antibody; Enigma homolog antibody; LIM antibody; PDLIM5 antibody; PDZ and LIM domain 5 antibody; PDZ and LIM domain protein 5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDLIM5 recombinant protein (Accession Number: NM_006457). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
NIH3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113631(PDLIM5 antibody) at dilution of 1:250. (Data provided by Angran Biotech (www.miRNAlab.com)).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113631(PDLIM5 Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113631(PDLIM5 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-PDLIM5 (IP:Catalog No:113631, 4ug; Detection:Catalog No:113631 1:300) with A549 cells lysate 2400ug.
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Background
PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression. This antibody is specific to PDLIM5, and its specificity has been confirmed by siRNA test.
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References
- Li Y, Gao Y, Xu Y. si-RNA-mediated knockdown of PDLIM5 suppresses gastric cancer cell proliferation in vitro. Chemical biology & drug design. 85(4):447-53. 2015.
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