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Product Name
PDLIM1/CLP36 antibody
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Description
PDLIM1/CLP36 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse lung tissue, human ileum tissue, human lung tissue, mouse heart tissue. Positive IF detected in MCF-7 cells. Positive IHC detected in human pancreas cancer tissue. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 36 kDa
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Tested applications
ELISA, IHC, IF, WB, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CLIM1 antibody; CLP 36 antibody; CLP36 antibody; Elfin antibody; hCLIM1 antibody; LIM domain protein CLP 36 antibody; PDLIM1 antibody; PDZ and LIM domain 1 antibody; PDZ and LIM domain protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDLIM1/CLP36 recombinant protein (Accession Number: NM_020992). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113629(PDLIM1,CLP36 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using PDLIM1 antibody Catalog No:113629 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:113629(PDLIM1,CLP36 Antibody) at dilution of 1:600
IP Result of anti-PDLIM1,CLP36 (IP:Catalog No:113629, 3ug; Detection:Catalog No:113629 1:600) with mouse heart tissue lysate 4000ug.
1X10^6 MCF-7 cells were stained with 0.2ug PDLIM1,CLP36 antibody (Catalog No:113629, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)
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References
- Miyazaki K, Ohno K, Tamura N, Sasaki T, Sato K. CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Experimental cell research. 318(14):1716-25. 2012.
- Hasegawa T, Ohno K, Funahashi S, Miyazaki K, Nagano A, Sato K. CLP36 interacts with palladin in dorsal root ganglion neurons. Neuroscience letters. 476(2):53-7. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"