-
Product Name
PDK2 antibody
- Documents
-
Description
PDK2 Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, HeLa cells, mouse skeletal muscle tissue, rat heart tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IHC detected in human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue, human skin tissue, human testis tissue. Observed molecular weight by Western-blot: 46 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
PDHK2 antibody; PDK2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PDK2 recombinant protein (Accession Number: NM_002611). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113625(PDK2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:113625(PDK2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113625(PDK2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PDK2 (IP:Catalog No:113625, 4ug; Detection:Catalog No:113625 1:500) with mouse skeletal muscle tissue lysate 2000ug.
-
Background
PDK2([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial) is also named as PDHK2 and belongs to the PDK/BCKDK protein kinase family. PDK2 plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDK2 is also involved in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. This protein has 2 isoforms produced by alternative splicing.
-
References
- Byun JK, Choi YK, Kang YN. Retinoic acid-related orphan receptor alpha reprograms glucose metabolism in glutamine-deficient hepatoma cells. Hepatology (Baltimore, Md.). 61(3):953-64. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"