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Product Name
PDIA6 antibody
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Description
PDIA6 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, L02 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse skin tissue. Positive IP detected in HEK-293 cells. Positive IHC detected in human stomach tissue, human spleen tissue, human stomach cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ER protein 5 antibody; ERP5 antibody; PDIA6 antibody; Protein disulfide isomerase A6 antibody; Protein disulfide isomerase P5 antibody; TXNDC7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDIA6 recombinant protein (Accession Number: NM_005742). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113682(PDIA6 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human stomach using Catalog No:113682(PDIA6 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach using Catalog No:113682(PDIA6 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PDIA6 (IP:Catalog No:113682, 4ug; Detection:Catalog No:113682 1:3000) with HEK-293 cells lysate 3000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:113682(PDIA6 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.
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References
- Zhu Z, Xu W, Dai J. The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis. The international journal of biochemistry & cell biology. 45(3):571-82. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"