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Product Name
PCNP antibody
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Description
PCNP Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lymphoma tissue. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, HEK-293 cells, Jurkat cells, PC-3 cells. Observed molecular weight by Western-blot: 24-28 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp781I24156 antibody; PCNP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PCNP recombinant protein (Accession Number: NM_001320395). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113745(PCNP antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113745(PCNP antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-PCNP (IP:Catalog No:113745, 4ug; Detection:Catalog No:113745 1:600) with HepG2 cells lysate 1800ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:113745(PCNP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PCNP represents PEST proteolytic signal containing nuclear protein
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"