-
Product Name
PCK2 antibody
- Documents
-
Description
PCK2 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in human brain tissue, human colon tissue, rat brain tissue. Observed molecular weight by Western-blot: 71kd
-
Tested applications
ELISA, WB, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
PCK2 antibody; PEPCK antibody; PEPCK M antibody; PEPCK2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PCK2 recombinant protein (Accession Number: NM_004563). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113740(PCK2 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113740(PCK2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113740(PCK2 antibody) at dilution of 1:100 (under 40x lens)
-
Background
PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"