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Product Name
PCK1 antibody
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Description
PCK1 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 67 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PCK1 antibody; PEPCK C antibody; PEPCK1 antibody; PEPCKC antibody; Phosphoenolpyruvate carboxykinase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PCK1 recombinant protein (Accession Number: NM_002591). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113739(PCK1 antibody) at dilution of 1:1000
IP Result of anti-PCK1 (IP:Catalog No:113739, 4ug; Detection:Catalog No:113739 1:500) with HepG2 cells lysate 3600ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:113739(PCK1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
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References
- Mauvoisin D, Wang J, Jouffe C. Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver. Proceedings of the National Academy of Sciences of the United States of America. 111(1):167-72. 2014.
- Liu M, Pan Q, Chen Y. Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy. Scientific reports. 5:11219. 2015.
- Guo F, Zhang Y, Su L, Ahmed AA, Ni Y, Zhao R. Breed-dependent transcriptional regulation of phosphoenolpyruvate carboxylase, cytosolic form, expression in the liver of broiler chickens. Poultry science. 92(10):2737-44. 2013.
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