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Product Name
PCBP2 antibody
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Description
PCBP2 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human cervical cancer tissue. Positive WB detected in HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Alpha CP2 antibody; hnRNP E2 antibody; HNRPE2 antibody; PCBP2 antibody; poly(rC) binding protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PCBP2 recombinant protein (Accession Number: NM_031989). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113685(PCBP2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113685(PCBP2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113685(PCBP2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Poly(rC)-binding protein 2 (PCBP2) is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC, It is also named Heterogeneous nuclear ribonucleoprotein E2, binds also poly(rU). It negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.(19881509) This antibody is a rabbit polyclonal antibody raised against a full-length human PCBP2 protein.
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References
- Chen Z, Zhang W, Ni L. Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury. Cellular and molecular neurobiology. 2015.
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