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Product Name
PAX4 antibody
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Description
PAX4 Mouse Monoclonal antibody. Positive WB detected in Hela cells, K-562 cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 38 kDa,58 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
KPD antibody; MODY9 antibody; paired box 4 antibody; Paired box protein Pax 4 antibody; PAX4 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of PAX4 recombinant protein (Accession Number: NM_006193). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107453(PAX4 antibody) at dilution of 1:500
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107453(PAX4 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
PAX4, a paired-box transcription factor, is a key regulator of pancreatic islet cell growth and differentiation. Differentiation of endoderm-derived endocrine pancreas is mediated through PAX4 and PAX6. PAX4 may act as a PAX6 repressor due to the competition for binding sites and lower transactivation potential of Pax-4. PAX4 has the potential to function as a tumor suppressor in human melanoma.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"