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Product Name
Parkin antibody
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Description
Parkin Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, C6 cells, SH-SY5Y cells. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AR JP antibody; LPRS2 antibody; PARK2 antibody; Parkinson disease protein 2 antibody; PDJ antibody; PRKN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Parkin recombinant protein (Accession Number: BC022014). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113585(Parkin Antibody) at dilution of 1:300
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:113585(Parkin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin–protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson’s disease. It has 6 isoforms produced by alternative splicing.
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