-
Product Name
PARD6B antibody
- Documents
-
Description
PARD6B Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, MCF-7 cells. Positive IHC detected in human pancreas tissue. Positive FC detected in HepG2 cells. Positive WB detected in HeLa cells, human placenta tissue, PC-3 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 50-53 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP, FC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
PAR 6 beta antibody; PAR 6B antibody; PAR6B antibody; PARD6B antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PARD6B recombinant protein (Accession Number: NM_032521). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113583(PARD6B antibody) at dilution of 1:500
IP Result of anti-PARD6B (IP:Catalog No:113583, 3ug; Detection:Catalog No:113583 1:200) with HEK-293 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:113583(PARD6B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:113583(PARD6B antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PARD6B antibody Catalog No:113583 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug PARD6B antibody (Catalog No:113583, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
-
Background
PARD6B represents par-6 partitioning defective 6 homolog beta (C. elegans)
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"