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Product Name
PACSIN3 antibody
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Description
PACSIN3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue, rat brain tissue. Positive FC detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human heart tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Endophilin I antibody; PACSIN3 antibody; SDPIII antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PACSIN3 recombinant protein (Accession Number: XM_047426856). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113480(PACSIN3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113480(PACSIN3 antibody) at dilution of 1:100 (under 25x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:113480(PACSIN3 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 MCF-7 cells were stained with 0.2ug PACSIN3 antibody (Catalog No:113480, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
PACSIN3 represents protein kinase C and casein kinase substrate in neurons 3
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"