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Product Name
PACSIN2 antibody
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Description
PACSIN2 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human skin tissue. Positive IF detected in NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, mouse heart tissue, mouse lung tissue. Positive IP detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 60-65 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
PACSIN2 antibody; SDPII antibody; Syndapin 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PACSIN2 recombinant protein (Accession Number: NM_001184970). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113479(PACSIN2 Antibody) at dilution of 1:600
IP Result of anti-PACSIN2 (IP:Catalog No:113479, 4ug; Detection:Catalog No:113479 1:500) with NIH/3T3 cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:113479(PACSIN2 Antibody) at dilution of 1:400 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:113479(PACSIN2 Antibody) at dilution of 1:400 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:113479(PACSIN2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).
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References
- Giridharan SS, Cai B, Vitale N, Naslavsky N, Caplan S. Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis. Molecular biology of the cell. 24(11):1776-90, S1-15. 2013.
- Simone LC, Caplan S, Naslavsky N. Role of phosphatidylinositol 4,5-bisphosphate in regulating EHD2 plasma membrane localization. PloS one. 8(9):e74519. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"