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Product Name
p70S6K antibody
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Description
p70S6K Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, Jurkat cells, rat spleen tissue. Positive IP detected in HeLa cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 70 kDa,90 kDa
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Tested applications
ELISA, WB, IHC, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
p70 alpha antibody; p70 ribosomal S6 kinase alpha antibody; p70 S6 kinase alpha antibody; p70 S6K 1 antibody; p70 S6K alpha antibody; p70 S6KA antibody; p70(S6K) alpha antibody; p70S6K antibody; P70S6K1 antibody; PS6K antibody; Ribosomal protein S6 kinase I antibody; RPS6KB1 antibody; S6K antibody; S6K beta 1 antibody; S6K1 antibody; STK14A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of p70S6K recombinant protein (Accession Number: NM_001272043). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113560(p70(S6K) antibody) at dilution of 1:1200
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113560(p70(S6K) antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113560(p70(S6K) antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-p70(S6K) (IP:Catalog No:113560, 3ug; Detection:Catalog No:113560 1:1000) with HeLa cells lysate 3000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:113560(p70(S6K) Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
1X10^6 HepG2 cells were stained with 0.2ug p70(S6K) antibody (Catalog No:113560, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway and it plays a key role in cell growth and proliferation by regulating insulin sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy.(PMID:22684641). It has 2 isoforms produced by alternative initiation.
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References
- Salhia B, Van Cott C, Tegeler T. Differential effects of AKT1(p.E17K) expression on human mammary luminal epithelial and myoepithelial cells. Human mutation. 33(8):1216-27. 2012.
- Ci Y, Shi K, An J. ROS inhibit autophagy by downregulating ULK1 mediated by the phosphorylation of p53 in selenite-treated NB4 cells. Cell death & disease. 5:e1542. 2014.
- Bai Y, Xuan B, Liu H, Zhong J, Yu D, Qian Z. Tuberous Sclerosis Complex Protein 2-Independent Activation of mTORC1 by Human Cytomegalovirus pUL38. Journal of virology. 89(15):7625-35. 2015.
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